Technical Data
A1012
Agarose DNA Fragments 50-2000bp (3:1 Blend)
25g
100g
250g
1Kg
Molecular Biology Storage: RTShipping: RT
Agarose for DNA Fragments 50-2000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.

Manufactured specifically for primer size fragments in the range of 50-2000 base pairs and exhibits exceptionally high transparency even at high concentrations (5%).

Specially purified to be free of background contamination that could interfere with restriction digests or ligations and selected for use in PCR product analysis.

Applications:
Analytical DNA Gels 2000bp
PCR products
Restriction Enzyme digestion products
Capillary Electrophoresis
Mutation Analysis
For use with all buffer systems

Recommended Usage (1X TAE):
Concentration Molecular Weight
1-4% 50-2000bp
1% 500-2000bp
2% 250-750bp
3% 125-500bp
4% 50-250bp
Note: A 3% agarose gel is equivalent to 8% polyacrylamide

Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4C for 1 hour before use.

CAS Number:
9012-36-6
Appearance:
White. granular, free flowing powder
EEO:
0.12
Absorbance (450nm,1.5%):
As reported
Melting Temperature:
93C
Gelling Temperature:
40.5C
Gel Strength (1.5%):
2000g/cm2
Gel Strength (4%):
4250g/cm2
pH in Solution (1.5%,H2O):
5.0-7.0
Loss on Drying:
7.0%
Sulfate:
0.1%
Ash:
0.35%
RNase:
None Detected
DNase:
None Detected
DNA Binding:
To Pass Test
Background Fluorescence (EtBr):
To Pass Test

Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.

Functional and Molecular Biology Testing: To Pass Test
Comparative assay of different size DNA fragments
Southern Blot (125-23,000bp)
Background Fluorescence (TAE/TE, EtBr):
DNA Binding (TAE, EtBr)
Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Ausubel, F.M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.