Agarose Low Melting
|BioSeparation?||Storage: RT/4°CShipping: RT|
Suitable for protein and large DNA separations 1000bp. The low melting temperature allows for the recovery, without damage, of nucleic acids below their denaturing temperatures.
The low melting point also ensures that the agarose will be in a liquid state where in-gel manipulations can be performed without prior purification of the DNA from the gel slice.
Tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
• Analytical DNA Gels 1,000bp
• Blotting Assays (Southern, northern)
• Restriction Enzyme Digestion
• Ligation• DNA Labeling• Nick Translation
• Random Priming• PCR• Tissue Culture
• Viral Plaque
• For use with all buffer systems
Recommended Usage (TBE):
Concentration Molecular Weight
pH (1.5%, Gel): As Reported
Melting Point (1.5%): 66°C
Gel Point (1.5%): 24-28°C
Gel Strength (1.5%): 500g/cm2
Clarity (1.5%): 40NTU
RNase: None Detected
DNase: None Detected
DNA Fragment Analysis: To Pass Test
Background Fluorescence: To Pass Test
Storage and Stability: Store at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
Form: White, homogeeous free flowing powder
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: Stewart, C.R. et al., (2009) J. Bacteriol. 191: 1537-1546. 1. Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989)