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Technical Data |
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A1030 |
Agarose High Gel Strength |
100g 250g 500g 1Kg |
| BioSeparation? | Storage: RTShipping: RT |
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High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications. Applications: • Pulsed Field Electrophoresis • Immunoelectrophoresis • Blotting Assays (Southern, northern) • Analytical DNA Gels 1000bp • Preparative Electrophoresis • For use with all buffer systems Recommended Usage (1X TAE): Concentration Molecular Weight 0.4-2.0% 50-500kb Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. CAS Number: 9012-36-6 |
pH (1.5%, Gel): As reported EEO: 0.135 Loss on Drying: 7.0% Melting Point (1.5%): 86-90°C Gel Point (1.5%): 34.5-37.5°C Gel Strength (1.5%): 3000g/cm2 Sulfate: 0.10% Ash: 0.25% Clarity: 40 NTU RNase: None Detected DNase: None Detected Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. Functional and Molecular Biology Testing: To Pass Test • Comparative assay of different size DNA fragments • Southern Blot (125-23,000bp) • Background Fluorescence (TAE/TE, EtBr): • DNA Binding (TAE, EtBr) • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Ausubel, F.M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).
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