		Technical Data
A1059-53D	Aggrecan (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)

Description:
Aggrecan is a proteoglycan component of cartilage matrix.

Applications:
Suitable for use in Immunohistochemistry, ELISA and Immunooprecipitation. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	3H524	Supernatant

Size		Storage	Shipping	Source	Host
2ml		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Human cartilage aggrecan.

Purity:

Tissue Culture Supernatant.

Form

Supplied as a liquid in 0.2M Tris HCl, pH 7.4, 5-10% fetal calf serum, 0.1% sodium azide.

Specificity:

Recognizes human aggrecan. Recognizes intact aggrecan under both reducing and non-reducing conditions in Western Blot. Does not recognize trypsin digested aggrecan.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.