Technical Data
A1059-53F
Aggrecan (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)
Description:
Aggrecan is a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal working dilutions to be determined by researcher.

Recommended Secondary Antibodies:
I1905-28A: IgM (X-Adsorbed) (HRP) Pab GtxMo
I1905-04A: IgM (X-Adsorbed) (PE) Pab GtxMo
I1905-18D: IgM (X-Adsorbed) (FITC) Pab GtxMo

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM3H526Supernatant
SizeStorageShippingSourceHost
2ml-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Purified human articular cartilage aggrecan
Purity:
Supernatant
Form
Supplied as a liquid in 0.2M Tris-HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
Specificity:
Recognizes a 205kD trypsin derived fragment of human Aggrecan. Species Crossreactivity: bovine. Does not crossreact with chicken, rat or fish
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Nicodemus, G. D. et al., (2011) Acta Biomaterialia 7: 492-504. 1. Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebreal cortex. J. Cell Mol. Med. 13: 3151-73. 2. Aigner, T. et al. (2002) Prognostic relevance of cell biologic and biochemical features in conventional chondrosarcomas. Cancer. 94: 2273-81. 3. Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-beta3 in Pellet Culture System Iranian J basic Med Res. 11: 10-17.