		Technical Data
A1059-53F	Aggrecan (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)

Description:
Aggrecan is a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Optimal working dilutions to be determined by researcher.

Recommended Secondary Antibodies:
I1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo
I1905-04A IgM (X-Adsorbed) (PE) Pab GtxMo
I1905-18D IgM (X-Adsorbed) (FITC) Pab GtxMo

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgM	3H526	Supernatant

Size		Storage	Shipping	Source	Host
2ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

Purified human articular cartilage aggrecan

Purity:

Supernatant

Form

Supplied as a liquid in 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.

Specificity:

Recognizes a 205kD trypsin derived fragment of human Aggrecan.Does not crossreact with chicken, rat or fish. Species Crossreactivity: bovine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

US Biological application reference: Nicodemus, G. D. et al., (2011) Acta Biomaterialia 7: 492-504. 1. Virgintino, D. et al. (2009) Differential distribution of aggrecan isoforms in perineuronal nets of the human cerebreal cortex. J. Cell Mol. Med. 13: 3151-73. 2. Aigner, T. et al. (2002) Prognostic relevance of cell biologic and biochemical features in conventional chondrosarcomas. Cancer. 94: 2273-81. 3. Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-beta3 in Pellet Culture System Iranian J basic Med Res. 11: 10-17.