Technical Data
A1059-53T
Aggrecan (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)
Description:
This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100.
Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin-embedded, alcohol-fixed frozen or un-fixed snap-frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k8J208Supernatant
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa-EPEEPFTFAPEI of human Aggrecan.
Purity:
Tissue culture supernatant
Form
Supplied as a liquid in 0.2M Tris/HCL, pH7.4, 1-10% fetal calf serum, 0.02% sodium azide.
Specificity:
Recognizes the linear amino acid sequence EPEEPFTFAPEI present in the interglobular domain (IGD) of human aggrecan. Species Crossreactivity: bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). Caterson B et al. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333-44 (2000).