Technical Data
Aggrecan ARGxx (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)
Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50 Detects a variety of epitopes between 50 and 250kD.
Immunohistochemistry: Formalin or paraformaldehyde- fixed paraffin embedded sections or alcohol-fixed frozen or unfixed snap-frozen sections.
Notes:Samples must be deglycosylated using 0.01 Units Chondroitinase ABC, 0.01 Units Keratanase and 0.0001 Units Keratanase II per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml4C (-20C Glycerol)Blue IceMouse
Not Determined
N-terminal neoepitope ARG after cleavage between aa EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).
Tissue culture supernatant
Supplied as a liquid in 0.2M Tris/HCl pH 7.4, 01-10% fetal calf serum, 40% glycerol.
Recognizes the aggrecanase (ADAMTS-1, -4, -5). Detects a variety of epitopes between 50 and 250kD. Species Crossreactivity: human, bovine, feline, canine, guinea pig, equine, porcine, rabbit, rat and sheep. Does not react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002).2. Caterson B et al. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333-44 (2000).