Technical Data
A1059-56
Agmatine (Guanidinium Analogue)
Description:
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:100 for 0.1-2.5% glutaraldehyde fixed tissues. Optimal fixation: 0.1-2.5% glutaraldehyde, 1% formalin. Minimum glutaraldehyde: 0.1%; works on paraffin embedded tissue (fixed with glutaraldehyde) but preferably epoxy embedded specifically developed for post-embedding protocols. Living, isolated goldfish retinas were maintained for 10 minutes in an oxygenated physiological solution that preserves normal neuronal activity under constant lighting. The medium also contained 10mM agmatine. After the incubation period retinas were fixed in a conventional 2.5% glutaraldehyde buffer and processed for immunohistochemistry using 250nm sections probed with a gold-labeled secondary antibody. The treated retinas were exposed to 125M kainate during the incubation. Kainate opens both AMPA and kainate selective ionotropic channels through which agmatine can enter cells.
Immunocytochemistry: On cells with glutamate-gated ion channels.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
2000Tests-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Agmatine (BSA coupled)
Purity:
Serum
Form
Supplied as a liquid in PBS, 1% goat serum, 0.05% thimerosal.
Specificity:
Recognizes Agmatine. Does not crossreact with arginine, glutamate or other amino acids. Is expected to recognize agmatine in mose vertebrate species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hille, B., Ionic channels of excitable membranes. Sinauer Assoc., Sunderland, MA (1984). 2. Li, et al., Science 263: (1994) 3. Quik, M., Brain Research 325: 7988 (1985). 4. Yoshikami, D., Science 212: 929930 (1985). 5. Marc, R.E., et al., J. Neuroscience 15: 51065129 (1995). 6. Marc, R.E., Society for Neuroscience Abstracts 21: 1992 #781.6 (1995). 7. Steullet, P., et al., J. Comparative Neurology 418: 270280 (2000).