Technical Data
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132aa in human) is normally expressed in skin. Its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.
Optimal dilutions to be determined by researcher.

Control Peptide:
A1059-58A: Agouti (Control Peptide)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceMouseRabbit
Synthetic peptied consisting of 21aa near the N-terminus of mouse Agouti (KLH).
Purified by immunoaffinity chromatography
Supplied as a liquid in PBS, 0.1% BSA.
Recognizes mouse Agouti. No homology is seen with AGRP, tubby, tub, TULP1 and TULP2. Species Sequence Homology: rat-90%, human, monkey-80%, porcine-73%, fox-75%, bovine-66%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miller, M.W., et al., Genes Develop. 7: 454 (1993). 2. Bultman. S.J., et al., Cell 71: 1195 (1992). 3. Kwon, H.Y., et al., PNAS 91: 97,601 (1994). 4. Wilson, B.D., Human Mol. Genet. 4: 223 (1995).