Technical Data
AGPAT6 (1-acyl-sn-glycerol-3-phosphate acyltransferase zeta, Testis spermatogenesis apoptosis-related protein 7, EC=, 1-AGP acyltransferase 6, 1-AGPAT 6, Lysophosphatidic acid acyltransferase-zeta, LPAAT-zeta)
AGPAT6 is a novel glycerolipid acyltransferase of the ER, which is crucial for the production of milk fat by the mammary gland.
Agpat6 deficient mice exhibit a 25% reduction in body weight and resistance to both diet-induced and genetically induced obesity. The reduced body weight is associated with increased energy expenditure, reduced triglyceride accumulation in BAT and WAT, reduced white adipocyte size, and lack of adipose tissue in the subdermal region. AGPAT6 plays a unique role in determining triglyceride content and composition in adipose tissue and liver that cannot be compensated by other members of the Agpat family. (1)

Cellular Localization: Membrane

Positive Control: Mouse skeletal muscle lysate

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
A synthetic peptide made to a C-terminal portion of the human AGPAT6 protein sequence (between residues 400-456). [Swiss-Prot Q86UL3]. Homology: 100% Hu, Mk
Purified by peptide immunoaffinity chromatography.
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
This antibody is specific for AGPAT6. Species Crossreactivity: Human and mouse. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beigneux, AP, et al. J. Lipid Res. 47: 734-744 (2006) 2. Vergnes, L., et al. J. Lipid Res. 47: 745-754 (2006)