Technical Data
A1059-60
Agrin
Description:
Applications:
Suitable for use n Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 2ug/ml
Immunocytochemistry: 2ug/ml
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q17Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat agrin (C-terminal construct). Epitope: near the Z splicing site.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1mM PMSF, 50% glycerol.
Specificity:
Recognizes agrin. Species crossreactivity: Rat and mouse. Binding of this antibody to agrin causes marked reduction in nAChR clusters.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hock et. al., EMBO J. (1994) 13:2814-2821.