Technical Data
Agrin (AGRN, FLJ45064, OTTHUMP00000044043)
Cellular Localization: Synaptic basal lamina at the neuromuscular junction.

Suitable for use in RID, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceRatMouse
Recombinant rat agrin, C-terminal construct. Epitope: Epitope is located near the 1st EGF-like repeat.
Mouse ascites
50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
Detects all isoforms of agrin. This antibody recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants. Species Crossreactivity: Crossreacts with Mouse and Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Grow WA & Gordon H Sialic acid inhibits agrin signaling in C2 myotubes. Cell Tissue Res 299:273-9 (2000). Hoch W Formation of the neuromuscular junction. Agrin and its unusual receptors. Eur J Biochem 265:1-10 (1999). Groffen AJ et al. Agrin is a major heparan sulfate proteoglycan in the human glomerular basement membrane. J Histochem Cytochem 46:19-27 (1998). Sharp AA & Caldwell JH Aggregation of sodium channels induced by a postnatally upregulated isoform of agrin. J Neurosci 16:6775-83 (1996). Campanelli JT et al. Alternative RNA splicing that determines agrin activity regulates binding to heparin and alpha-dystroglycan. Development 122:1663-72 (1996). Glass DJ et al. Agrin acts via a MuSK receptor complex. Cell 85:513-23 (1996). Bowen DC et al. Neural agrin activates a high-affinity receptor in C2 muscle cells that is unresponsive to muscle agrin. J Neurosci 16:3791-7 (1996). Hoch W et al. Structural domains of agrin required for clustering of nicotinic acetylcholine receptors. EMBO J 13:2814-21 (1994). Ferns MJ et al. The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron 11:491-502 (1993). Tsim KW et al. cDNA that encodes active agrin. Neuron 8:677-89 (1992). Wallace BG Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus. J Neurosci 9:1294-302 (1989).