Technical Data
A1059-60G
Agrin (AGRN, FLJ45064, OTTHUMP00000044043)
Description:
Cellular Localization: Synaptic basal lamina at the neuromuscular junction.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J213Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat agrin, C-terminal construct. Epitope: Epitope has been mapped near the 2nd laminin domain homology region and causes a marked reduction in the number of nicotinic acetylcholine receptor (nAChR) clusters. Insertion of an 8aa peptid
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.1mM PMSF.
Specificity:
Detects all isoforms of agrin. Species Crossreactivity: Crossreacts with Mouse and Rat. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Khan AA et al. Physiological regulation of the immunological synapse by agrin. Science 292:1681-6 (2001). Hoch W Formation of the neuromuscular junction. Agrin and its unusual re