Cellular Localization: Synaptic basal lamina at the neuromuscular junction.
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Recombinant rat agrin, C-terminal construct. Epitope: Epitope has been mapped near the 2nd laminin domain homology region and causes a marked reduction in the number of nicotinic acetylcholine receptor (nAChR) clusters. Insertion of an 8aa peptid|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.1mM PMSF.|
|Detects all isoforms of agrin. Species Crossreactivity: Crossreacts with Mouse and Rat. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Khan AA et al. Physiological regulation of the immunological synapse by agrin. Science 292:1681-6 (2001). Hoch W Formation of the neuromuscular junction. Agrin and its unusual re|