Suitable for use in Immunocytochemistry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Cellular Localization: Synaptic basal lamina at the neuromuscular junction.
Positive Control: Mouse Brain Tissue Extract.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Recombinant rat agrin, C-terminal construct. Binds to an epitope close to splicing site Z and causes a marked reduction in the number of nicotinic acetylcholine receptor(nAChR) clusters in samples from mouse and rat origins.|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 0.1mM PMSF, 50% glycerol.|
|Recognizes agrin. Species Crossreactivity: Crossreacts with Mouse and rat. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Khan AA et al. Physiological regulation of the immunological synapse by agrin. Science 292:1681-6 (2001). Hoch W Formation of the neuromuscular junction. Agrin and its unusual receptors. Eur J Biochem 265:1-10 (1999). Hoch W et al. Structural domains of agrin required for clustering of nicotinic acetylcholine receptors. EMBO J 13:2814-21 (1994). Ferns MJ et al. The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron 11:491-502 (1993). Tsim KW et al. cDNA that encodes active agrin. Neuron 8:677-89 (1992). Wallace BG Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus. J Neurosci 9:1294-302 (1989). |