Technical Data
A1059-62
AGRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)
Description:
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1). However, recombinant Agouti protein also antagonizes the MC2R and MC4R. AGRP (132 aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus. AGRP levels are increased several folds in ob/ob mice. AGRP is a strong antagonist of MC3R and MC4R. Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide (A1059-62A) per well.
Western blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 15aa from mouse AGRP conjugated to KLH. Species Sequence Homology (85%): human, bovine. No homology seen with Agouti, tubby, tub, TULP1 or TULP2.
Purity:
Purified by immunoaffinity chromotography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes mouse AGRP. Epitope: C-terminal
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shutter, R.J. et al (1997) Genes Develop. 11:593. 2. Ollmann M.M. et al (1997) Science 278:135