Technical Data
A1059-62B
AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)
Description:
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Direct): 0.5-1.0ug/ml well. The detection limit for recombiant human AgRP is ~6ng/ml.
Western Blot: 1-2ug/ml. The detection limit for recombinant human AgRP is ~25ng/lane under non-reducing and reducing conditions using colorimetric detection system.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b9H47Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to human Agouti-Related Protein, expressed in Sf 21 cells (NP_015531).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile 1ml sterile PBS.
Specificity:
Recognizes human AgRP. Shows ~10% crossreactivity with recombinant mouse AgRP in direct ELISA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.