Technical Data
A1059-62D
AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)
Description:
Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1). However, recombinant Agouti protein also antagonizes the MC2R and MC4R. AGRP (132 aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus. AGRP levels are increased several folds in ob/ob mice. AGRP is a strong antagonist of MC3R and MC4R. Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000.
ELISA: 1:10,000-100,000.
Optimal dilutions to be determined by the researcher.

Control Peptide: A1059-62A AGRP, Mouse Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to 15aa sequence near C-terminus of mouse AGRP (KLH).
Purity:
Serum
Form
Supplied as a liquid, PBS, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript. Species sequence homology: human and bovine (85%). No homology is seen with Agouti, tubby, tub, TULP1 and TULP2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shutter RJ et al (1997) Genes Develop. 11, 593; Ollmann MM et al (1997) Science 278, 135.