Technical Data
A1059-62E
AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)
Description:
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein
(AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1). However, recombinant Agouti protein also antagonizes the MC2R and MC4R. AGRP (132 aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus. AGRP levels are increased several folds in ob/ob mice. AGRP is a strong antagonist of MC3R and MC4R. Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.

Applications:
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000
Western Blot: 0.1ug/ml with human brain lysates gave bands at ~45 and 24kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CRKLGTAMNPCSRT, from the C Terminus of human AGRP (NP_001129.1; NP_015531.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Specificity:
Recognizes human Agouti-Related Protein (AGRP). Species Sequence Homology: canine and porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Sarruf, D.A. et al., (2009) Endocrinology 150:707-712. 1. Breen TL, Conwell IM, Wardlaw SL., Effects of fasting, leptin, and insulin on AGRP and POMC peptide release in the hypothalamus. Brain Res. 2005 Jan 25;1032(1-2):141-8. General references: 1. Shutter RJ et al (1997) Genes Develop. 11, 593; Ollmann MM et al (1997) Science 278, 135.