Technical Data
A1059-62L
AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (capture): 1ug/ml using 100ul/well.
Western Blot: 1-2ug/ml Detection limit for recombinant human AgRP and recombinant mouse AgRP is ~5ng/lane under non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Matched Pair:
Capture: A1059-62L
Detection: A1059-62M

Hybridoma:
Myeloma with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H48Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Sf 21-derived, recombinant human Agouti-Related Protein.
Purity:
Purified by Protein G affinity chromatography. Endotoxin: 0.1EU/ug (LAL).
Form
Supplied as a lyophilized powder from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile 1ml PBS.
Specificity:
Recognizes human AgRP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.