Technical Data
A1059-63A
Agouti-Related Protein (AGRP)
Description:
The Agouti-related protein (Agrp) -- secreted hypothalamic factors regulated by the metabolic state in mammals -- is involved in energy homeostasis and plays an important role in the control of feeding behavior. Agouti protein and Agouti-related protein are paracrine-signaling molecules that normally regulate pigmentation and body weight. The mature human protein is a single polypeptide chain of 112 amino acid residues consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. Circular dichroism studies indicated that the purified protein contains primarily random coil and beta-sheet secondary structure. The hypothalamic expression of Agrp is regulated by leptin, and overexpression of Agrp in transgenic animals results in obesity and diabetes.

Applications:
Suitable for use in Indirect ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Amino Acid Sequence;
MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to 128aa with a N-teminal His-tag from human Agouti-related protein expressed in E. coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 50ul sterile ddH2O.
Specificity:
Recognizes human Agouti-Related Protein (AGRP) at ~14.4kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mizuno TM, Mobbs CV affei M., Halaas J., Ravussin E. et al.: Hypothalamic agouti-related protein messenger ribonucleic acid is inhibited by leptin and stimulated by fasting. Endocrinology; 140(2):814-7 (1999) 2. Ollmann, MM, Lamoreux, M , Wilson, BD, Barsh, GS: Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev; 12(3):316-30, (1998) 3. Song, Y (8), Golling, G, Thacker, TL, Cone, RD (24)Agouti-Related Protein (AGRP) Is Conserved and Regulated by Metabolic State in the Zebrafish, Danio rerio. Endocrine; 22(3):257-66 (2003)