		Technical Data
A1059-63A	Agouti-Related Protein (AGRP)

Description:
The Agouti-related protein (Agrp) -- secreted hypothalamic factors regulated by the metabolic state in mammals -- is involved in energy homeostasis and plays an important role in the control of feeding behavior. Agouti protein and Agouti-related protein are paracrine-signaling molecules that normally regulate pigmentation and body weight. The mature human protein is a single polypeptide chain of 112 amino acid residues consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. Circular dichroism studies indicated that the purified protein contains primarily random coil and beta-sheet secondary structure. The hypothalamic expression of Agrp is regulated by leptin, and overexpression of Agrp in transgenic animals results in obesity and diabetes.

Applications:
Suitable for use in Indirect ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Amino Acid Sequence;
MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Recombinant protein corresponding to 128aa with a N-teminal His-tag from human Agouti-related protein expressed in E. coli.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 50ul sterile ddH2O.

Specificity:

Recognizes human Agouti-Related Protein (AGRP) at ~14.4kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mizuno TM, Mobbs CV affei M., Halaas J., Ravussin E. et al.: Hypothalamic agouti-related protein messenger ribonucleic acid is inhibited by leptin and stimulated by fasting. Endocrinology; 140(2):814-7 (1999) 2. Ollmann, MM, Lamoreux, M , Wilson, BD, Barsh, GS: Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev; 12(3):316-30, (1998) 3. Song, Y (8), Golling, G, Thacker, TL, Cone, RD (24)Agouti-Related Protein (AGRP) Is Conserved and Regulated by Metabolic State in the Zebrafish, Danio rerio. Endocrine; 22(3):257-66 (2003)