Technical Data
A1059-69D
AHA1 (Activator of Hsp90 ATPase 1)
Description:
Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3).
Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k10F681Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~1.59mg/ml
Immunogen:
Mouse Aha1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M Sodium chloride pH7.2.
Specificity:
Recognizes Mouse Aha1. Species Crossreactivity: human,
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387(pt.3):789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) JCell Sci.121(pt.5):717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4):1188-1197.