Technical Data
A1059-78B
AIF (IN) (AIF, MGC111425, Programmed cell death protein 8, mitochondrial precursor, PDCD8)
Description:
AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-1ug/ml

Optimal working dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Peptide corresponding to amino acids 517-531 of human AIF.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes apoptosis inducing factor (AIF). Reacts with an internal region of AIF. Species Crossreactivity: Human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Susin, S.A., et al. (1999). Molecular characterization of mitochondrial apoptosis - inducing factor. Nature. 397: 441-6.