		Technical Data
A1059-78C	AIF (NT) (AIF, MGC111425, Programmed cell death protein 8, mitochondrial precursor, PDCD8)

Description:
AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:500. Non-reducing conditions only.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Highly Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~5mg/ml

Immunogen:

Peptide corresponding to amino acids 109 - 122 of human AIF (NT).

Purity:

Purified IgG was prepared from whole serum by ion exchange chromatography.

Form

Supplied as a liquid in PBS with 0.02% sodium azide.

Specificity:

Recognizes apoptosis inducing factor (AIF). The antibody reacts with the N-terminal region of the mature protein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Susin. S.A. et al. (1999). Molecular characterization of mitochondrial apoptosis - inducing factor. Nature. 397: 441-6.