		Technical Data
A1059-78T	AIF (Apoptosis-inducing Factor 1 Mitochondrial, Apoptosis-inducing Factor Mitochondrion-associated 1, AIFM1, COXPD6, MGC111425, Programmed Cell Death Protein 8, PDCD8)

Description:
Apoptosis-inducing factor (AIF) is a phylogenetically ancient mitochondrial intermembrane flavoprotein. AIF induces caspase-independent peripheral chromatin condensation and large-scale DNA fragmentation when added to purified nuclei. Upon apoptosis induction AIF translocates to the cytosol and the nucleus.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 0.25-1µg/ml
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human, mouse, rat heart lysate, or K562 cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to human AIF.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human AIF. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Marzo I. et al. FEBS Lett 427(2):198-202 (1998). 2. Susin S.A., et al. Nature. Feb 4;397(6718):441-6 (1999). 3. Cande C., et al. Biochimie 84(2-3):215-222 (2002).