Technical Data
A1059-86Z
AIF1, Recombinant, Human, aa1-147, His-Tag (Allograft Inflammatory Factor 1, AIF-1, DASS-82G15.2, IBA1, IRT-1, Em:AF129756.17, Interferon gamma Responsive Transcript, Ionized Calcium-binding Adapter Molecule)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
AIF1 (Allograft inflammatory factor 1) is induced by cytokines and interferon. This protein is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. AIF1 is a cytoplasm, calcium-binding protein whose expression in transplanted human hearts correlates with rejection and development of coronary artery vasculopathy (CAV).

Source:
Recombinant corresponding to aa1-147 from human AIF1, fused to His-tag at N-terminus expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MSQTRDLQGG KAFGLLKAQQ EERLDEINKQ FLDDPKYSSD EDLPSKLEGF KEKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKKLI GEVSSGSGET FSYPDFLRMM LGKRSAILKM ILMYEEKARE KEKPTGPPAK KAISELP

Molecular Weight:
~18.9kD (167aa), confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18.9kD
Source: E. coli
Purity: ~95% (SDS-PAGE)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 2mM DTT, 200mM sodium chloride, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kelemen S.E., et al (2005) Am. J. Pathol. 167:619-626. 2. Schulze J.O., et al. (2008) FEBS J. 275:4627-4640.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.