Technical Data
A1061
AHSP, Recombinant Human (Alpha-hemoglobin-stabilizing protein, EDRF, ERAF, Erythroid associated factor, Erythroid differentiation-related factor)
100ug
Molecular Biology Storage: -70CShipping: Dry Ice
AHSP (Alpha-hemoglobin stabilizing protein), also known as ERAF (Erythroid associated factor), is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of aplha-hemoglobin during normal erythroid cell development. It specifically protects free alpha-hemoglobin from precipitation in live cells and in solution. This protein is downregulated in transmissible spongiform encephalopathies (TSEs). It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Recombinant AHSP protein, expressed in E. coli and purified by using conventional chromatography techniques.

Source:
Recombinant corresponding to human AHSP, 1aa-102, expressed in E. coli.

AA Sequence:
MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Molecular Weight:
11.8kD
Source: E.coli
Purity: ~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
Concentration: ~1mg/ml
Form: Supplied as liquid in 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Dos Santos CO., et al. (2008) J Biol Chem. 283(40):26956-64. 2. Sekijima Y., et al. (2005) Cell. 121(1):73-85.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.