Technical Data
A1099-01A
Ajuba
Description:
Ajuba (an Urdu word meaning “curiosity”) is a 55-60kD member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha- catenin:F-actin binding, beta-catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF- B activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538 amino acids (aa) in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa1-335), an NES (aa280 -287), and three LIM domains that bind multiple proteins (aa336-397, 401-461 and 462-530). Ajuba is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:20,000-1:80,000.
Western Blot: 1:500-1:2500. a 57kD band is seen.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~2mg/ml
Immunogen:
Synthetic peptide: CQESRHSYPPALGSPG, corresponding to amino acids 224-239 of Human Ajuba.
Purity:
Purified by immunoaffinity chromatography.
Form
0.15M Sodium Chloride with 0.02M Potassium Phosphate [pH 7.2] containing 0.01% sodium azide
Specificity:
This antibody detects a strong band at ~57kD that we believe corresponds to Ajuba. It also detects other bands but we note that other Ajuba antibodies, particularly that used in Hirota T et al. Cell 114:585-598, require IP before Western for successful use. We hope to further test this antibody using IP in due course. Species Crossreactivity: Expected to crossreact with Human (100% identity with immunogen), Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Hirota T et al. Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells. Cell 114:585-98 (2003).