Technical Data
A1102-80
AKAP, Muscle (mAKAP, A-Kinase Anchor Protein)
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-4ug/ml of this lot detected mAKAP in RIPA cell lysates from L6 cells.Rat L6 cell lysate was resoved by elecrophoresis, transferred to nitrocellulose and probed with A1102-80 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated mAKAP from an L6 RIPA cell lysate.
Immunohistochemistry: Has been shown to detect mAKAP in differentiated rat neonatal ventriculocytes and frozen rat heart tissue.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
His-tagged, recombinant protein corresponding to residues 1666-2319 of human mAKAP (muscle AKAP).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, 30% glycerol..
Specificity:
Recognizes human mAKAP, Mr ~255kD, in skeletal muscle, ~300kD in brain and heart. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kapiloff, M.S., et al., J. Cell Sci. 112: 2725-2736, 1999.