Technical Data
A1102-80A
AKAP3 (A Kinase (PRKA) Anchor Protein 3, AKAP110, Fibrousheathin 1, FSP95, PRKA3, SOB1, Sperm Oocyte Binding Protein 1)
Description:
A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.

Cellular Localization: Localizes to the ribs of the fibrous sheath in the principal piece of the sperm tail.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: 1-3ug/ml. Predicted molecular weight: 95kD. detects a band of ~ 90kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: TPLQLLDWLMVNL, representing the C-terminus of the human protein (residues 841-853) according to NP_006413.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Species Crossreactivity: Tested: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lefevre A et al. Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition. Biochem Biophys Res Commun 259:60-6 (1999).