		Technical Data
A1102-80A	AKAP3 (A Kinase (PRKA) Anchor Protein 3, AKAP110, Fibrousheathin 1, FSP95, PRKA3, SOB1, Sperm Oocyte Binding Protein 1)

Description:
A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.

Cellular Localization: Localizes to the ribs of the fibrous sheath in the principal piece of the sperm tail.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000.
Western Blot: 1-3ug/ml. Predicted molecular weight: 95kD. detects a band of ~ 90kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide: TPLQLLDWLMVNL, representing the C-terminus of the human protein (residues 841-853) according to NP_006413.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Specificity:

Species Crossreactivity: Tested: Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Lefevre A et al. Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition. Biochem Biophys Res Commun 259:60-6 (1999).