Technical Data
A1102-80E
AKAP3 (A-kinase Anchor Protein 3, AKAP-3, A-kinase Anchor Protein 110kD, AKAP 110, Protein Kinase A-anchoring Protein 3, AKAP110, Cancer/Testis Antigen 82, CT82, Fibrous Sheath Protein of 95kD, FSP95, Fibrousheathin I, Fibrousheathin-1, Protein Kinase A-a
Description:
A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
ELISA: >1:32,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human testes

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to TPLQLLDWLMVNL, from the C-terminus of the protein sequence according to NP_006413.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human AKAP3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lefevre A, Duquenne C, Rousseau-Merck MF, Rogier E, Finaz C. Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition. Biochem Biophys Res Commun. 1999 May 27;259(1):60-6. PMID: 10334916.