		Technical Data
A1102-80F	AKAP3 (A-kinase Anchor Protein 3, AKAP-3, A-kinase Anchor Protein 110kD, AKAP 110, Protein Kinase A-anchoring Protein 3, AKAP110, Cancer/Testis Antigen 82, CT82, Fibrous Sheath Protein of 95kD, FSP95, Fibrousheathin I, Fibrousheathin-1, Protein Kinase A-a

Description:
A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in human testis lysates on ~90kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-TPLQLLDWLMVNL, from human AKAP3, at C-terminal (NP_006413.2).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human AKAP3. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lefevre A, Duquenne C, Rousseau-Merck MF, Rogier E, Finaz C. Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition. Biochem Biophys Res Commun. 1999 May 27;259(1):60-6.