Technical Data
A1102-83
AKAP9 (A-kinase Anchor Protein 9, AKAP-9, A-kinase Anchor Protein 350kD, AKAP 350, hgAKAP 350, A-kinase Anchor Protein 450kD, AKAP 450, CG-NAP, Protein Hyperion, Protein Kinase A-anchoring Protein 9, AKAP 120-like Protein, Centrosome- and Golgi-localized
Description:
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in many isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cell line lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CSGSTTQFHAGMR, from the C-terminus of the protein sequence according to NP_005742.4; NP_671714.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes AKAP9. Species sequence homology: mouse and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.