Technical Data
A1102-83L
AKAP10 (A-kinase Anchor Protein 10, Mitochondrial, AKAP-10, Dual Specificity A Kinase-anchoring Protein 2, D-AKAP-2, Protein Kinase A-anchoring Protein 10, PRKA10)
Description:
Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 0.3-1ug/ml, observed in rat brain lysates on ~74kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-RKVKGKEQEKTSD, from rat AKAP10, at the internal region of the protein (NP_009133.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes rat AKAP10. Species sequence homology: Bovine, canine, human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Burns-Hamuro LL, Barraclough DM, Taylor SS. Identification and functional analysis of dual-specific A kinase-anchoring protein-2. Methods Enzymol. 2004;390:354-74.