Technical Data
AKAP 95 (A Kinase Protein 95)
A-kinase proteins (AKAPs) function to localize the cAMP-dependent protein kinase (PKA) to specific subcellular sites. Anchoring of PKA is an efficient means to adapt the kinase for specific cAMP-responsive events as it permits rapid and preferential phosphorylation of target substrates upon elevation of cAMP. So far, 35 unique AKAPs have been identified which function to tether the kinase to a variety of cellular structures and organelles. AKAP95 is a 95kD protein which is a component of the nuclear matrix in all cell-lines.

Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml. Detects AKAP95 in human A431 lysates and HeLa nucler extract.
Immunocytochemistry: 10ug/ml. Detect AKAP95 in human A431 cells.
Optimal dilution determined by the researcher.

Included Positive Antigen Control:
H1838: HeLa nuclear extract cell lysate.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
HI1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ug-20CBlue IceRabbit
Recombinant mouse AKAP expressed in E. coli
Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Recognizes AKAP 95. Species Crossreactivity: human, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Coghlan, et. al., J. Biol. Chem. 269: 7658-7665 (1994).