Technical Data
A1105-25
AKAP 150 (A Kinase Protein 150)
Description:
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 detects AKAP 150 in a rat brain cytosolic preparation (5ug). Rat brain cytosolic preparation (5ug/lane) was resolved by electrophoresis, transferred to nitrocellulose and probed with A1105-25. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
200ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa428-449, C-TTVGQAEEATVGQAEEATVGQA of rat AKAP 150 (KLH).
Purity:
Serum
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes rat AKAP 150, Mr 150kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Colledge, M., et al., Neuron 27: 107119, 2000