Technical Data
A1105-75A
AKAP-associated Sperm Protein (ASP, ROPN1L, Ropporin-1 Like, AKAP-associated Sperm Protein)
Description:
AKAPs act as scaffolding proteins co-ordinating phosphotase:kinase signaling at the cytoskeleton. They interact with PKAs and anchor them to specific sub-cellular compartments and participate in multiple cellular processes including growth, metabolism and differentiation. ASP, a 26KD protein, is a novel AKAP-interacting protein. It interacts with the amphipathic helix of AKAP3 and facilitates AKAP3-PKA interactions. It consists of a conserved N-terminal AKAP-binding domain and a central cysteine-rich Fz domain. It is specifically expressed in the sperms and might play a significant role in sperm motily and also facilitate cytoskeletal reorganization during spermiogenesis.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa58-73(KDRMEMPTATQKTDTG) and 176-191(ARLDSDVSPLETESYL) of human ASP.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human AKAP-associated sperm protein (ASP). Species sequence homology: mouse, bovine, chimpanzee, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Carr,D.W., Fujita,A., Stentz,C.L., Liberty,G.A., Olson,G.E. and Narumiya,S. Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. J. Biol. Chem. 276 (20), 17332-17338 (2001). 2. Anway,M.D., Ravindranath,N., Dym,M. and Griswold,M.D. Identification of a murine testis complementary DNA encoding a homolog to human A-kinase anchoring protein-associated sperm protein. Biol. Reprod. 66 (6), 1755-1761 (2002).