		Technical Data
A1105-75A	AKAP-associated Sperm Protein (ASP, ROPN1L, Ropporin-1 Like, AKAP-associated Sperm Protein)

Description:
AKAPs act as scaffolding proteins co-ordinating phosphotase:kinase signaling at the cytoskeleton. They interact with PKAs and anchor them to specific sub-cellular compartments and participate in multiple cellular processes including growth, metabolism and differentiation. ASP, a 26KD protein, is a novel AKAP-interacting protein. It interacts with the amphipathic helix of AKAP3 and facilitates AKAP3-PKA interactions. It consists of a conserved N-terminal AKAP-binding domain and a central cysteine-rich Fz domain. It is specifically expressed in the sperms and might play a significant role in sperm motily and also facilitate cytoskeletal reorganization during spermiogenesis.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa58-73(KDRMEMPTATQKTDTG) and 176-191(ARLDSDVSPLETESYL) of human ASP.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

Specificity:

Recognizes human AKAP-associated sperm protein (ASP). Species sequence homology: mouse, bovine, chimpanzee, canine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Carr,D.W., Fujita,A., Stentz,C.L., Liberty,G.A., Olson,G.E. and Narumiya,S. Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. J. Biol. Chem. 276 (20), 17332-17338 (2001). 2. Anway,M.D., Ravindranath,N., Dym,M. and Griswold,M.D. Identification of a murine testis complementary DNA encoding a homolog to human A-kinase anchoring protein-associated sperm protein. Biol. Reprod. 66 (6), 1755-1761 (2002).