Technical Data
A1124-01N
AKT (Rac PKa, PKBa)
Description:
The protein kinase B (PKB, also called AKT) family of protein kinases plays a key role in insulin signaling, cellular survival, and transformation. AKT1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to region surrounding Threonine 308 from AKT.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes AKT. Species Crossreactivity: human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hemmings, B. et al. Science 275, 628 (2001). 2. Jones, P. et al. Proc Natl Acad Sci USA 88, 4171 (1991). 3. Bellacosa, A. et al. Science 254, 274 (1991). 4. Maira, S. et al. Science 294, 374 (2002). 5. Stephens, L. et al. Science 279, 710 (1998). 6. Alessi, D. et al. Curr Biol 7, 261 (1997).