Technical Data
A1125-01L
Akt1, active, Recombinant, Human (Rac PKa, PKBa)
10ug
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Technical Data |
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A1125-01L |
Akt1, active, Recombinant, Human (Rac PKa, PKBa) |
10ug |
| Molecular Biology | Storage: -70°CShipping: Dry Ice |
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Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system. Specific Activity: ~12pmol/min/ug Assay Conditons: Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. Molecular Weight: 60.2kD |
Source: Sf9 insect cells Purity: 70% (SDS-PAGE) Concentration: ~0.2mg/ml Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3mM DTT, 50% glycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Kuijl C, et al., Nature. 2007 Nov 29;450 (7170):725-30. 2. Vogiatzi P and Giordano A. Cancer Biol Ther. 2007 Aug 3;6(10).
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