Technical Data
Akt1, active, Recombinant, Human, GST-Tag (Rac PKa, PKBa)
Molecular Biology Storage: -70CShipping: Dry Ice
Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system.

Specific Activity:

Assay Conditons:
Enzyme reaction is conducted in a buffer
containing 50mM HEPES, pH 7.5, 10mM
magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour.

Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Molecular Weight:
Source: Sf9 insect cells
Purity: 70% (SDS-PAGE)
Concentration: ~0.2mg/ml
Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3mM DTT, 50% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kuijl C, et al., Nature. 2007 Nov 29;450 (7170):725-30. 2. Vogiatzi P and Giordano A. Cancer Biol Ther. 2007 Aug 3;6(10).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.