Technical Data
A1125-19B
Akt1, (Rac PKa, PKBa), phosphorylated (Thr308)
Description:
Akt1/PKBa is a Ser/Thr protein kinase, whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3, 4). Three mammalian Akt1/PKBa isoforms have been identified.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5,000 -10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9E459Serum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A phospho specific peptide corresponding to residues surrounding Threonine 308 of human Akt1.
Purity:
Serum
Form
Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes Akt1 phosphorylated at Threonine 308. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Burgering, B.M. and P.J. Coffer.Nature 376: 599–602, 1995.2. Cardone, M.H., et al. Science 282: 1318–1321, 1998.3. Hajduch, E., et al. FEBS Lett. 492: 199–203, 2001.4. Cross, D.A., et al. Nature 378: 785-789, 1995.