Technical Data
Alamar Blue™
Biochemicals Storage: -20°CShipping: Blue Ice
Alamar Blue™ is a safe, nontoxic aqueous dye that is used to assess cell viability and cell proliferation and is supplied as a sterile indigo colored liquid. Alamar Blue has also been shown to be a rapid and simple non-radioactive assay alternative to the [3H] thymidine incorporation assay.

Suitable for use in Cell Viability Assessment and Cell Proliferation Assessment.

Storage and Stability:
May be stored at 4°C, -20°C or -70°C. When stored frozen, it is important to warm to 37°C upon thawing and mixed well to be assured of complete resolubilization. Protect from light. Storage at RT under lighted conditions adversely affects its absorbance properties.
Cell Viability Assessment:
The innate metabolic activity of cells can be monitored with Alamar Blue™. In the presence of added toxic compounds such as antimicrobial agents, this innate metabolic activity ceases. Procedures which cite the use of Alamar Blue™ in assays of efficacy of antimicrobial agents call for the determination of Alamar Blue™ reduction at a single predetermined time point.

Cell Proliferation Assessment:
Alamar BlueTM is soluble, stable in culture medium and is non-toxic. The continuous monitoring of cells in culture is therefore permitted. Specifically, Alamar Blue™ does not alter the viability of cells cultured for various times as monitored by Trypan Blue exclusion. Cells grown in the presence of Alamar Blue™ and subsequently analyzed by Flow Cytometry for CD44, CD45RB, CD4 and heat stable antigen are found to produce similar numbers of viable cells and antigen expressing cells as non- Alamar Blue™ exposed cells. Because Alamar Blue™ is non-toxic, the cells under study can be returned to culture or used for other purposes including histological studies. Proliferation measurements with Alamar Blue™ may be made either spectrophotometrically by monitoring the absorption of Alamar Blue™ supplemented cell culture media at two wavelengths. Alternatively, proliferation measurements with Alamar Blue™ may be made fluorometrically.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Zhou, C. et al., (2008) Br J Pharmacol. 154:440–450. 2. Perale, G. et al., (2008) International Journal of Applied Ceramic Technology 5:37-43. 3. Zhu Y., et al. 2014. Chinese Chemical Letters. 25: 693-698.
Other References: 1. Ahmed, S.A., et al (1994) A new rapid and non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H] thymidine incorporation assay. J Immunol Meth 170:211-224. 2. Fields, R.D. and M.V. Lancaster (1993) Dual-attribute continuous monitoring of cells proliferation/cytotoxicity. American Biotechnology Laboratory 11(4): 48-50. 3. Goegan. P., et al (1995) Effects of serum protein and colloid on the alamarBlue assay in cell cultures. Toxic In Vitro 9: 257-266. 4. Ishiyama, M., et al (1996) A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, Neutral Red and crystal violet. Biol. Pharm. Bull. 19(11):1518-1520. 5. Lancaster, M.V. and R.D. Fields (1992) User defined, colorimetric antimicrobial susceptibility assays. American Clinical Laboratories, April 1992. 6. Nociari, M.M., et al (1998) A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity. J Immunol Meth 213(2): 157-167. 7. Pagé, B., et al (1993) A new fluorimetric assay for cytotoxicity measurements in vitro. Intl J Oncol 3: 473-479. 8. Alley, M.C., et al. (1988). Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay. Cancer Research 48: 589-601. 9. William, H.H., et al. (1965). Ultraviolet and Visible Absorption Methods, p94-95, Instrumental Methods of Analysis, D. Van Nostrand Co. Inc., Princeton, N.J.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.