Technical Data
A1180
Alamar Blue™
25ml
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Technical Data |
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A1180 |
Alamar Blue™ |
25ml |
| Biochemicals | Storage: -20°CShipping: Blue Ice |
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Alamar Blue™ is a safe, nontoxic aqueous dye that is used to assess cell viability and cell proliferation and is supplied as a sterile indigo colored liquid. Alamar Blue has also been shown to be a rapid and simple non-radioactive assay alternative to the [3H] thymidine incorporation assay. Applications: Suitable for use in Cell Viability Assessment and Cell Proliferation Assessment. Storage and Stability: May be stored at 4°C, -20°C or -70°C. When stored frozen, it is important to warm to 37°C upon thawing and mixed well to be assured of complete resolubilization. Protect from light. Storage at RT under lighted conditions adversely affects its absorbance properties. |
Cell Viability Assessment: The innate metabolic activity of cells can be monitored with Alamar Blue™. In the presence of added toxic compounds such as antimicrobial agents, this innate metabolic activity ceases. Procedures which cite the use of Alamar Blue™ in assays of efficacy of antimicrobial agents call for the determination of Alamar Blue™ reduction at a single predetermined time point. Cell Proliferation Assessment: Alamar BlueTM is soluble, stable in culture medium and is non-toxic. The continuous monitoring of cells in culture is therefore permitted. Specifically, Alamar Blue™ does not alter the viability of cells cultured for various times as monitored by Trypan Blue exclusion. Cells grown in the presence of Alamar Blue™ and subsequently analyzed by Flow Cytometry for CD44, CD45RB, CD4 and heat stable antigen are found to produce similar numbers of viable cells and antigen expressing cells as non- Alamar Blue™ exposed cells. Because Alamar Blue™ is non-toxic, the cells under study can be returned to culture or used for other purposes including histological studies. Proliferation measurements with Alamar Blue™ may be made either spectrophotometrically by monitoring the absorption of Alamar Blue™ supplemented cell culture media at two wavelengths. Alternatively, proliferation measurements with Alamar Blue™ may be made fluorometrically. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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US Biological application references: 1. Zhou, C. et al., (2008) Br J Pharmacol. 154:440–450. 2. Perale, G. et al., (2008) International Journal of Applied Ceramic Technology 5:37-43. 1. Ahmed, S.A., et al (1994) A new rapid and non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H] thymidine incorporation assay. J Immunol Meth 170:211-224. 2. Fields, R.D. and M.V. Lancaster (1993) Dual-attribute continuous monitoring of cells proliferation/cytotoxicity. American Biotechnology Laboratory 11(4): 48-50. 3. Goegan. P., et al (1995) Effects of serum protein and colloid on the alamarBlue assay in cell cultures. Toxic In Vitro 9: 257-266. 4. Ishiyama, M., et al (1996) A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, Neutral Red and crystal violet. Biol. Pharm. Bull. 19(11):1518-1520. 5. Lancaster, M.V. and R.D. Fields (1992) User defined, colorimetric antimicrobial susceptibility assays. American Clinical Laboratories, April 1992. 6. Nociari, M.M., et al (1998) A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity. J Immunol Meth 213(2): 157-167. 7. Pagé, B., et al (1993) A new fluorimetric assay for cytotoxicity measurements in vitro. Intl J Oncol 3: 473-479. 8. Alley, M.C., et al. (1988). Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay. Cancer Research 48: 589-601. 9. William, H.H., et al. (1965). Ultraviolet and Visible Absorption Methods, p94-95, Instrumental Methods of Analysis, D. Van Nostrand Co. Inc., Princeton, N.J.
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