Technical Data
D-Alanine, Conjugated
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: Indirect
Immunohistochemistry: 1:7500 to detected D-Alanine immunoreactive cell bodies located in the lateral and ventro-lateral thalamus of paraformaldehyde/glutaraldehyd fixed and frozen rat brain.
Optimal dilutions to be determined by the researcher.

Using a conjugate D-Alanine-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio (a)
D-Alanine-PC 1
L-Alanine-PC 1/> 50,000
Glycine-PC 1/> 50,000
D-Valine-PC 1/> 50,000
D-Leucine-PC 1/> 50,000
(a) : D-Alanine-PC concentration/other conjugated amino acid concentration at half displacement;

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

50ug-20CBlue IceRabbit
Synthetic D-Alanine conjugated to a protein carrier
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Recognizes protein conjugated D-Alanine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
KIM HG, KIM NR, GIM MG, LEE JM, LEE SY, KO MY, KIM JY, HAN SH, CHUNG DK. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J. Immunol. (2008) 180(4):2553-61.