Technical Data
A1206-05
D-Alanine, Conjugated
Description:
Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000-1:15,000
Immunohistochemistry: 1:7500. D-Alanine immunoreactive cell bodies located in the lateral thalamus of rat brain. Also, Immunoreactive cell bodies containing D-Alanine in the ventro-lateral thalamus of rat brain.

Specificity:
Using a conjugate D-Alanine-Glutaraldehyde-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

Compound Cross-reactivity ratio (a)

D-Alanine-G-BSA 1
L-Alanine-G-BSA 1/> 50,000
Glycine-G-BSA 1/> 50,000
D-Valine-G-BSA 1/> 50,000
D-Leucine-G-BSA 1/> 50,000

(a) : D-Alanine-G-BSA concentration/unconjugated or conjugated aminoacids concentration at half displacement.
G = Glutaraldehyde.

Recommended dilution
The anti-conjugated b-alanine antibodies can be diluted between 1/2,000-1/10,000.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic D-Alanine conjugated to BSA.
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes protein conjugated D-Alanine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.