Technical Data
Alanine Aminotransferase (ALT or SGPT) BioAssay™ Kit
Kits and Assays Storage: -20°CShipping: RT
Alanine aminotransferase (ALT), also called serum glutamic pyruvic transaminase (SGPT), is a transaminase (EC or alanine transaminase. ALT is found in serum and in various body tissues, but is usually associated with the liver. It catalyzes the reaction:
-Ketoglutarate + Alanine Glutamate + Pyruvate

It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In this ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to -ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color ( max = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10mU per well.

Kit Components:
A1210-22A: ALT Asay Buffer, 1x25ml
A1210-22B: OxiRedTM (in DMSO), 1x200ul
A1210-22C: ALT Enzyme Mix, 1x1vial. Lyophilized
A1210-22D: ALT Substrate, 1x1vial. Lyophilized
A1210-22E: Pyruvate Standard, 1x100ul. Supplied at a concentration of 100nmol/ul
A1210-22F: ALT Positive Control, 1x1vial. Lyophilized

Storage and Stability:
Store all kit components at -20°C, protected from light. Unopened kit components are stable for up to 6 months at -20°C.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.