Technical Data
Alanine Transaminase Assay Kit, BioAssay™
Kits and Assays Storage: -20°CShipping: Blue Ice
Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage.

Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery. ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340nm is proportional to ALT activity.

Key Features:
•Sensitive. Linear detection range: 2–100U/L
•Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 minutes.

Direct Assays: ALT activity in serum, plasma and other biological samples.
Drug Discovery/Pharmacology: Effects of drugs on ALT activity.

Kit Components:
A1210-23A: Assay Buffer, 1x24ml
A1210-23B: LDH, 1x120ul
A1210-23C: Cosubstrate, 1x600ul
A1210-23D: NADH, 1x1vial

Storage and Stability:
Store at -20°C. Stable for 6 months after receipt. A1210-23D 3 weeks after reconstitution. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Bergmeyer H.U., et al. (1978). Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clin. Chem. 24(1):58-73. 2. Siest G. et al. (1975). Aspartate aminotransferase and alanine aminotransferase activities in plasma: statistical distributions, individual variations, and reference values. Clin Chem. 21(8):1077-87. 3. Liu L. et al. (2008). Expression, purification, and initial characterization of human alanine aminotransferase (ALT) isoenzyme 1 and 2 in High-five insect cells. Protein Expr Purif. 60(2):225-231.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.