Technical Data
A1274-90H
Albumin, Mouse Serum (HRP)
Description:
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoelectrophoresis. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:50,000
Western Blot: 1:500-1:15,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceMouseGoat
Concentration:
~0.5mg/ml
Immunogen:
Full length mouse Albumin
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% BSA, 0.1% Pro-Clean 400, before the addition of glycerol to 40%. Labeled with Horseradish Peroxidase (HRP).
Specificity:
Recognizes mouse Albumin. May react with serum albumins from other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.