		Technical Data
A1275-06B	Albumin, Bovine Serum (BSA)

Description:
Bovine serum albumin (BSA) is the most abundant protein in bovine plasma. BSA is produced in the liver and the concentration of BSA in plasma is 35-50mg/ml. The molecular mass is 69kD (1).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 Ag14. Female CF1 x Balb c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	6F10	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Bovine	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Bovine serum albumin isolated from bovine plasma.

Purity:

Purified by Protein A/G affinity chromatography from culture supernatant.

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes bovine serum albumin (BSA). No crossreactivity with rabbit serum albumin. Reacts with HSA, but only a quarter as strong as the reaction with BSA. Crossreactivity with other species not tested. A strong reaction is seen in ELISA with bovine serum albumin coated directly onto the microtiter well. In Western blot after SDS-PAGE, reacts with bovine albumin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Scott T & Eagleson M Concise Encyclopedia Biochemistry: Walter de Gruyter, New York. (1988).