Technical Data
A1275-06B
Albumin, Bovine Serum (BSA)
Description:
Bovine serum albumin (BSA) is the most abundant protein in bovine plasma. BSA is produced in the liver and the concentration of BSA in plasma is 35-50mg/ml. The molecular mass is 69kD (1).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 Ag14. Female CF1 x Balb c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6F10Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceBovineMouse
Concentration:
~0.5mg/ml
Immunogen:
Bovine serum albumin isolated from bovine plasma.
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes bovine serum albumin (BSA). No crossreactivity with rabbit serum albumin. Reacts with HSA, but only a quarter as strong as the reaction with BSA. Crossreactivity with other species not tested. A strong reaction is seen in ELISA with bovine serum albumin coated directly onto the microtiter well. In Western blot after SDS-PAGE, reacts with bovine albumin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scott T & Eagleson M Concise Encyclopedia Biochemistry: Walter de Gruyter, New York. (1988).