Technical Data
A1275-25X
Albumin, Human Serum (BSA) (BSA & Azide Free)
Description:
Applications:
Suitable for use in the recognition of HSA in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the detection or conjugate (label) antibody in sandwich immunoassay. Matched to A1275-24 as the capture or solid phase antibody.

Hybridoma:
Hybridization of SP2/0 myeloma cells with spleen cells from Balb/c mice.

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a2B6Affinity Purified
SizeStorageShippingSourceHost
250ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~2mg/ml (Sigma protein assay kit)
Immunogen:
Human Serum Albumin (HSA)
Purity:
Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
Form
Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins added. No preservatives added. Also available with sodium azide. See A1275-25.
Specificity:
Human serum albumin. No crossreactivity with other human protein. Does not react with bovine serum albumin, egg white albumin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.