Technical Data
A1325-20A
Albumin, Bovine Serum (BSA, ALB, Bos d 6, MGC179277)
Description:
The defined antibody specificity is directed to albumin as tested against bovine sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine serum and the purified albumin. The cytochemical grade of allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as capture antibody and detection reagent; in Western blotting. This product is not pre-diluted.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:1000-1:10000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceBovineRabbit
Concentration:
Immunogen:
Highly purified albumin isolated from bovine serum.
Purity:
Purified by DEAE chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O. Dilute further with PBS, pH 7.2.
Specificity:
Recognizes bovine Albumin. Species crossreactivity: feline, canine, goat, guinea pig, hamster, equine, human, monkey, mouse, rat, sheeo, porcine. Does not react with chicken, duck, pigeon or rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.