Technical Data
A1327-49
Albumin, Human Serum (HSA) (HRP)
Description:
Albumin is synthesized in the liver and consists of a single polypeptide chain with a molecular weight of 66kD. It is present in all body fluids and is most abundant plasma protein. It acts as a transport protein for long chain fatty acids, prostaglandins, hematin, thyroxin, copper II, cystine and pyridoxal phosphate amongst many other substances that bind more weakly. The average serum concentration of albumin is 42g/L.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:50,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store lyophilized product at 4C if to be used immediately within two weeks; store at -20C for long-term storage. Upon reconstitution, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human Serum Albumin
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml polyethylene glycol-8000. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, purified human serum albumin IgG and human serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Read, J.D. et al., (2007) Applied and Environmental Microbiology 73:5088-5096.