Technical Data
A1334-33L
ALDH1A1, ID (Retinal Dehydrogenase 1, RalDH1, RALDH 1, Aldehyde Dehydrogenase Family 1 Member A1, Aldehyde Dehydrogenase, Cytosolic, ALHDII, ALDH-E1, ALDH1A1, ALDC, ALDH1, PUMB1)
Description:
ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:50
Western Blot: 1:1000
Immunohistochemistry: 1:10-1:50
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 302-331 in the central region of human ALDH1A1, UniProt Accession #P00352. Species sequence homology: monkey, mouse
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human ALDH1A1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moore,S., J Stud Alcohol Drugs 68(2), 192-196 (2007). 2. Collard,F., Biochimie 89(3), 369-373 (2007).