Technical Data
ALDH3A1, Recombinant, Human (Aldehyde Dehydrogenase, Dimeric NADP-preferring, ALDHIII, Aldehyde Dehydrogenase 3, Aldehyde Dehydrogenase Family 3 Member A1, ALDH3)
Molecular Biology Storage: -20CShipping: Blue Ice
Aldehyde Dehydrogenases (ALDHs) are NAD(P)+- dependent enzymes that catalyze the oxidation of endogeneously produced and exogeneous aldehydes to their corresponding acids (1). They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and in lipid peroxidation. ALDH3A1 is also known as stomach aldehyde dehydrogenase. It exists as a homodimer, and prefers NADP+ over NAD+ as its co-factor (2). It preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates (3). The enzyme is highly expressed in stomach and cornea (2, 4). In the cornea, its proposed roles have been to absorb UV-light, reduce oxidative damage, maintain corneal refractive and transparence properties, and display chaperone-like activity (4, 5). It also has been identified as a lung cancer biomarker (6).

Recombinant corresponding to Ser2-His453 of human Aldehyde Dehydrogenase, fused with 6-His-tag at N-terminal, expressed in E. coli.

Molecular Weight:

Endotoxin Level:
1EU/1ug (LAL)

Biological Activity:
Measured by the ability to catalyze the oxidation of 4-nitrobenzaldehyde. The specific activity is >6000 pmol/min/ug, as measured under the described conditions.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
Source: E.coli
Purity: ~95% (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in Tris, sodium chloride, DTT, glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Marchitti, S. A. et al. (2007) Pharmacol. Rev. 59:125. 2.Yin, S. J. et al. (1991) FEBS Lett. 283:85. 3.Hsu, L. C. et al. (1992) J. Biol. Chem. 267:3030. 4.Estey, T. et al. (2007) Exp. Eye Res. 84:3. 5.Estey, T. et al. (2010) PLOS One. 5:e15218. 6. Kim, B. et al. (2007) Cancer Res. 67:7431.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.